Looking at this figure
A titration analysis is warranted to evaluate how well Buscar performs as single-cell population sizes decrease when computing scores. This also raises the question of whether pooling is the appropriate strategy. On one hand, pooling captures the full extent of cellular heterogeneity across replicates, ensuring that all biological variation present within wells is incorporated into the calculation. However, pooling also alters the underlying distribution in morphological feature space, as features that are sparse within individual wells may become more prominent when combined with data from other replicates. As a result, pooling does not simply improve estimation but can fundamentally change the distribution being compared, complicating the interpretation of Buscar scores.
Looking at this figure
A titration analysis is warranted to evaluate how well Buscar performs as single-cell population sizes decrease when computing scores. This also raises the question of whether pooling is the appropriate strategy. On one hand, pooling captures the full extent of cellular heterogeneity across replicates, ensuring that all biological variation present within wells is incorporated into the calculation. However, pooling also alters the underlying distribution in morphological feature space, as features that are sparse within individual wells may become more prominent when combined with data from other replicates. As a result, pooling does not simply improve estimation but can fundamentally change the distribution being compared, complicating the interpretation of Buscar scores.